![]() abyssi (primary source)." See Lynch et al., 2015 doi: 10.1073/pnas.1514974112 link Supplementary Materials p.1 right column bottom paragraph:"For most prokaryotes, there is just a single origin of replication (ORI) per genome, and mean Okazaki fragment lengths are on the order of 1 to 2 kb as there are many thousands of such fragments per genome, it is clear that the vast majority of the costs associated with primer preparation and ligation are associated with lagging strands. Because DNA polymerases lack de novo DNA synthesis activity, each Okazaki fragment contains an RNA-DNA primer at its 5-end, and this primer is synthesized with low fidelity by primase-DNA pol complex (5, 7). "Subsequently, the lagging strand polymerase transits from the 3'-OH terminus of the just completed Okazaki fragment to the new primer terminus, resuming DNA synthesis through 1000–2000 nt in E. The average size of Okazaki fragments in eukaryotic cells is 150200 nucleotides (nt). et al., Identification of short 'eukaryotic' Okazaki fragments synthesized from a prokaryotic replication origin. A protocol for quantitative genome-wide detection of replication initiation, fork progression and termination, based on purification and strand-specific sequencing of Okazaki fragments isolated. Marinov, The bioenergetic costs of a gene, PNAS. p.1611 left column top paragraph PubMed ID 15184548 Average length of Okazaki fragment 165 bp Budding yeast Saccharomyces cerevisiae Michael Lynch and Georgi K. The replication-related organization of bacterial genomes. Decreasing the DnaG concentration to 30 nM as in previously described experiments resulted in a gap spacing of 4.3☐.6 kb, an increase of 1.7-fold. Escherichia coli 1,000 - 2,000: Pyrococcus abyssi ~100 nts Indeed, our observed spacing at 300 nM DnaG is 2.5☐.4 kb, approximately twice the average Okazaki fragment length at that primase concentration (12 kb Tougu and Marians, 1996). ![]()
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